Preliminary Separation, Purification and Determination of Flavonoids in Aurea Helianthus

Authors: Ri Hyon Il1, Kim Ryu Song2, Kang Myong Su2, Yang Jong Hyok 1, Kim Yu Il1

Abstract :The extraction, separation and purification of flavonoids from Aurea Helianthus were performed using various organic solvents and gradient elution methods. Ultraviolet spectrophotometry and high performance liquid chromatography-mass spectrometry were used to analyze flavonoids. Results:UV-Vis spectrophotometer UV 2450 (SHIMADZU) was used to preliminarily identify the content of flavonoids in the extracts of Aurea Helianthus, flavonoid content in the ethyl acetate extract was the highest ,48.3±0.3%.There was a good linear relationship between 0.004 and 0.024 mg/ml (R2 = 0.998).Ethyl acetate extract was used as the test object for high performance liquid chromatography-mass spectrometry analysis.In the linear range 10.0-240μg,good precision and reproducibility, stable within 6 hours.The average recoveries of quercetin,Hyperoside, and rutin were 105.32%, 102.25%, and 103.14%.The content of rutin in the gradient elution (EtOAc:MeOH=4:1) was 42.8±0.3%, and the content of hyperin and quercetin in the gradient elution (EtOAc) were 51.2±0.4% and 12.5 ±0.3%. The gradient effluent (EtOAc:MeOH=4:1, EtOAc) had a total flavonoid content of 84.2±0.8% and 75.3±0.9%.Conclusion:Conclusion: This method is simple, rapid, and highly sensitive.It can be used to extract, isolate, purify and determine the content of flavonoids in Aurea Helianthus.

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[v1] 2018-06-28 09:04:20

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