Authors: Muvvala Subrahmanya Sudhir1, Ratnakaram Venkata Nadh
A simple, precise, accurate, high reproducible and economical visible spectrophotometric method of analysis for the synthesized rasagiline hemitartrate was developed and validated. The proposed method involves diazotization of sulphanilic acid under acidic conditions in presence of sodium nitrite, followed by its coupling with rasagiline in alkaline medium. The absorption spectra of the yellow colored chromophore formed between rasagiline and positive diazonium ion has absorption maximum at 440 nm. The linear regression analysis data for the calibration plot showed good linear relationship (r = 0.99937) with in the concentration range of 0 – 10 μg mL-1. The limit of detection and limit of quantitation were found to be 0.033 μg mL-1and 0.1 μg mL-1 respectively. This method was tested and validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is accurate, precise and reproducible (R.S.D. < 2 %).
Comments: 8 Pages. OK
Download: PDF
[v1] 2019-05-19 05:37:40
Unique-IP document downloads: 71 times
Vixra.org is a pre-print repository rather than a journal. Articles hosted may not yet have been verified by peer-review and should be treated as preliminary. In particular, anything that appears to include financial or legal advice or proposed medical treatments should be treated with due caution. Vixra.org will not be responsible for any consequences of actions that result from any form of use of any documents on this website.
Add your own feedback and questions here:
You are equally welcome to be positive or negative about any paper but please be polite. If you are being critical you must mention at least one specific error, otherwise your comment will be deleted as unhelpful.