Production of L-methionine by Cornybacterium glutamicum MTCC 2745 was assessed in batch fermentation by varying parameters viz., initial glucose concentration, initial nitrogen concentration, fermentation time, temperature, pH, initial shaking rate. Nynhydrin test and paper chromatography were used to identify methionine. Methionine estimated by using nitroprusside method. Effect of nitrogen sources on methionine production was examined. The parameters such as concentrations of glucose, ammonium sulphate, K2HPO4, MgSO47H2O, 3,4-dihydroxy benzoic acid and Yeast extract are optimized and maximum yield was 5.6 g of methionine/L.
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[v1] 2019-05-19 05:51:53
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