Biochemistry

1808 Submissions

[7] viXra:1808.0239 [pdf] submitted on 2018-08-16 07:54:00

Biofield Energy Healing Based Vitamin D3: An Improved Overall Bone Health Activity in MG-63 Cell Line

Authors: Karen Byrnes Allen, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Comments: 8 Pages.

The potential of The Trivedi Effect®-Consciousness Energy Healing vitamin D3 and DMEM medium was investigated in human bone osteosarcoma cells (MG-63). The Test Items (TI) i.e. vitamin D3 and DMEM were separated into two parts. Bone health parameters were tested such as Alkaline Phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Karen Byrnes Allen and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the Untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 71% with safe and nontoxic profile among test samples on MG-63 cell line. The level of ALP was significantly increased by116.4% at 50µg/mL in the UT-DMEM+BT-TI group, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respectively in the BT-DMEM+UT-TI group. In addition, ALP level was increased by 42.3% and 90.6% at 10 and 50 μg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated group. The level of collagen was significantly increased by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively in the UT-DMEM+BT-TI group, while 22.6% and 190.6% at 0.1 and 1µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 140.6% and 39.9% at 0.1 and 1 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 128.7% at 50 μg/mL, in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μg/mL, respectively as compared with the untreated group. Thus, the study results concluded that The Trivedi Effect® Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.
Category: Biochemistry

[6] viXra:1808.0238 [pdf] submitted on 2018-08-16 07:54:36

Improved Overall Bone Health Activity in MG-63 Cell Line after Biofield Energy Healing Treatment on Vitamin D3.

Authors: Kathleen Starr Vagt, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Comments: 10 Pages.

The aim of the study is to evaluate the role of The Trivedi Effect®-Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health parameters in vitro using MG-63 cells. The parameters of bone health tested are alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Kathleen Starr Vagt and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 72% with safe and nontoxic profile among test samples on MG-63 cell line. ALP was significantly increased by 140.8% (50 µg/mL), 167.3% (100 µg/mL),and 223.5% (50 µg/mL) in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated group. The level of collagen was significantly increased by 120.7% and 93% at 1 and 10 µg/mL, respectively in the UT-DMEM+BT-TI group, while 46.7%, 100.9%, and 29.2% at 1, 10, and 50 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 60.9%, 93%, and 14.4% at 1, 10, and 50 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 95.9% at 100 µg/mL in the UT-DMEM+BT-TI group, while 162% and 35.5% at 50 and 100 µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 57% and 107.4% at 50 and 100 µg/mL, respectively as compared with the untreated group.T hus, the study results concluded that The Trivedi Effect Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.
Category: Biochemistry

[5] viXra:1808.0237 [pdf] submitted on 2018-08-16 07:55:06

An Alternative Therapeutic Approach on Bone Cells Differentiation after Administration of Biofield Energy Treated Vitamin D3 in MG-63 Cell line

Authors: Eileen Mary Meagher, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Comments: 6 Pages.

The objective of the current experiment was to evaluate the potential of Consciousness Energy Healing-based test items (Vitamin D3 and DMEM) on bone parameters. Both the TI were distributed into two individual parts. One part of each samples was received Consciousness Energy Healing Treatment by Eileen Mary Meagher and those samples were labelled as the Biofield Treated (BT) samples, while the other parts of each samples were denoted as the untreated test items (UT). Cell viability data showed more than 71% viable cells signifies a safe and nontoxic profile of test items on MG-63 cell line. ALP was significantly increased by 132.18% and 126.52% in BT-DMEM+UT-TI and BT-DMEM+BT-TI, respectively at 50 µg/mL; while 139.06% (at 0.1 µg/mL) in UT-DMEM+BT-TI compared to untreated. Collagen was significantly increased by 172.76% at 1 µg/mL in UT-DMEM+BT-TI, while 89.65% in BT-DMEM+UT-TI at 10 µg/mL than untreated. Additionally, collagen was significantly increased by 111.2% in BT-DMEM+UT-TI at 50 µg/mL, while 113.16% in BT-DMEM+BT-TI at 100 µg/mL than untreated. The percent of bone mineralization was significantly increased by 206.21%, 168.74%, and 224.82% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 0.1 µg/mL compared with the untreated. In addition, percent of bone mineralization data showed a significant increased by 190.63%, 181.07%, and 245.41% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 1 µg/mL than untreated. Moreover, at 10 µg/mL it was significantly increased by 129.44%, 111.76%, and 109.81% in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI, respectively at 10 µg/mL with respect to untreated. Based on the study outcomes it emphasized that Biofield Energy Treatment could regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of autoimmune diseases.
Category: Biochemistry

[4] viXra:1808.0079 [pdf] submitted on 2018-08-06 13:29:38

Project Lucinda / Project Adam/Awan

Authors: Salvatore Gerard Micheal
Comments: 2 Pages.

homo sapiens is a divine extra-special project called Project Adam/Awan
Category: Biochemistry

[3] viXra:1808.0045 [pdf] submitted on 2018-08-04 02:48:56

Lab Report

Authors: Nilantha Gamalathge
Comments: 19 Pages.

Abstract Bifidobacteria Identification is important in medical and pharmaceutical industry. The molecular analytic technique such as PCR gel electrophoresis and MALDI –TOF-MS are high sensitive and useful technique in identifying Bifidobacteria. This practical was planned to identification of Bifidobacteria by both method. PCR method is based on denaturing, annealing and extension of target DNA in the bacteria in vitro with other requirements like taq polymerase enzyme, DNA primers, Mg etc with temperature control. MALDI –TOF-MS is based on analyet Matrix co crystallization and firing of laser beam get ionization and detection time of flight by mass spectrometry Un known 3 cutler plats (S1, S2,S3) were used for both methods and correctly identified up to genus level by PCR agarose gel electrophoresis. MALDI TOF MS was done in direct application and formic acid extraction method. Both ways identified up to bifid bacteria species and lactobacillus. Both methods were equally highly sensitive to identify Bifidobacteria. Identification of species was highly accurate MALDY TOF –MS method. But both methods have done with limited sample size. Both methods are complex, high cost and need high technical knowledge. MALDI method results depend on software apart from other steps. PCR can improve to identify Bifid bacteria up to species level with the use of species-level primers.MALDI method with formic acid exaction can improve sensitivity with the purification of the protein. (PCR-polymerase Chain Reaction.MALDI-TOF-MS- Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry).
Category: Biochemistry

[2] viXra:1808.0003 [pdf] submitted on 2018-08-01 05:13:31

Improved Structural and Functional Integrity of Bone Health Parameters After Treatment with Consciousness Energy Treated Vitamin D3

Authors: Debra Jane Schnitzer, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Comments: 9 Pages.

The study objective was to investigate the effect of Consciousness Energy Healing-based vitamin D3 and DMEM medium on bone health. The test items (vitamin D3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Debra Jane Schnitzer and those samples were denoted as the Biofield Energy Treated (BT) samples, while the other parts of each sample were referred as the untreated test items (UT). Parameters such as ALP, collagen, and bone mineralization were performed to evaluate the bone strength in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT cell viability assay. ALP was significantly increased by 27.75%, 28.21%, and 60% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the UT-DMEM + UT-Test item group. Moreover, the ALP level was significantly raised by 11.17% and 74.57% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 100 µg/mL compared to the UT-DMEM + UT-Test item group. Collagen was significantly increased by 54.53% and 115.01% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item groups, respectively at 0.1 µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 131.87% (at 1 µg/mL) and 179.77% (at 10 µg/mL) in the UT-DMEM + BT-Test item group compared to the untreated group. Apart from this, the percent of bone mineralization was distinctly increased by 75.94%, 125.79%, and 117.38% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 10 µg/mL compared to the untreated group. Additionally, the percentage of bone mineralization was significantly increased by 46.08%, 173.22%, and 171.17% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50 µg/mL compared to the untreated group. Altogether, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be an alternative approach for nutraceutical supplement to combat vitamin D3 deficiency and able to fight against various bone-related disorders including rickets, low bone density, osteomalacia, bone and joint pain, bone fractures, osteoporosis, osteoma, osteogenesis imperfecta, Paget’s disease, deformed bones, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.
Category: Biochemistry

[1] viXra:1808.0002 [pdf] submitted on 2018-08-01 05:14:07

In Vitro Effects of Biofield Energy Treated Vitamin D3 Supplementation on Bone Formation by Osteoblasts Cells

Authors: Sakina Aleemah Ansari, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Comments: 8 Pages.

Various bone disorders including fractures, significant pain and height loss, disability to stand up, and walk occurs due to insufficient level of vitamin D and calcium in the body. Both plays an important role to improve the patients' quality of life with respect to bone disorders. The present experimental analysis designed to explore the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on various bone health parameters such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. Both the test items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. Each sample received the Consciousness Energy Healing Treatment by Sakina Aleemah Ansari and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Cell viability using MTT assay showed that cell viability was more than 79% with safe and nontoxic profile on MG-63 cell line. The level of ALP was significantly increased by 119% (at 50 µg/mL), 161.5% (at 1 µg/mL), and 252.3% (at 50 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. Collagen level was significantly increased by 81% (at 10 µg/mL), 117.9% (at 1 µg/mL), and 134.2% (at 0.1 µg/mL) in UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared with the untreated test item and DMEM group. In addition, the percent of bone mineralization was significantly increased by 235.2% at 100 µg/mL in UT-DMEM+BT-TI group, while 152.7% and 92.6% at 0.1 and 1 µg/mL, respectively in BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 158% at 1 µg/mL as compared with the untreated group. Overall, the experimental data suggested that the Biofield Energy Treated vitamin D3 and DMEM would play an important role in the promotion and maintenance of strong and healthy bones, which improve quality of life. Biofield Energy Treatment might also regulates the osteoblast function, improves bone mineralization, and calcium absorption in wide range of bone disorders along with wide range of adverse health conditions, comprising cancer and certain autoimmune diseases.
Category: Biochemistry