Biochemistry

1902 Submissions

[11] viXra:1902.0247 [pdf] submitted on 2019-02-15 03:36:58

Investigation of Biofield Treated Vitamin D3 to Improve Immunity of Bone Cells in MG-63 Cell Line.

Authors: Inthirani Arul, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Sambhu Charan Mondal, Snehasis Jana
Comments: 10 Pages.

The study was aimed to evaluate the effect of Consciousness Energy Healing-based vitamin D3 and DMEM on bone health in human bone osteosarcoma cells (MG-63). Test items (vitamin D3 and DMEM), were divided into two parts. One part of each sample was received the Biofield Energy Treatment by Inthirani Arul and those samples were denoted as Biofield Treated (BT) samples, while other parts of each sample were referred as untreated test items (UT). Cell viability revealed that test samples were found as safe in tested concentrations. ALP was significantly increased by 260% in BT-DMEM + BT-TI group at 0.1 µg/mL compared to UT-DMEM + UT-TI group. Moreover, collagen level was significantly increased by 102.94% and 52.94% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 0.1µg/mL than UT-DMEM + UT-TI group. Further, collagen level was significantly increased by 114.94%, 114.03%, and 152.33% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 1µg/mL than untreated. Besides, percent of bone mineralization was significantly enhanced by 82.95% and 119.55% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 10µg/mL than untreated. Moreover, it was significantly increased by 166.44% and 212.49% in BT-DMEM + UT-TI and BT-DMEM + BT-TI groups, respectively at 50µg/mL than untreated. It was also significantly increased by 104.44%, 100%, and 230.22% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 100µg/mL than untreated. Among three combination regimens, the Biofield Treated vitamin D3 and DMEM group was comparatively more improved the bone-related parameters and might be useful against various bone-related disorders (rickets, low bone density, osteomalacia, osteoporosis, Paget’s disease, chondrodystrophia fetalis), stress management, anti-aging, autoimmune and inflammatory disorders near future.
Category: Biochemistry

[10] viXra:1902.0157 [pdf] submitted on 2019-02-09 00:12:48

Wound Healing Potential of Biofield Energy Treatment in HaCaT Cell Line Using Scratch Assay

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Comments: 7 Pages.

Wounds may be acute, subacute, or chronic and are the common clinical life entities. It is a complex biochemical process to restore the cell structures that depends upon cell proliferation and migration, mainly by keratinocytes and fibroblast cells. The aim of the present study was to evaluate the effect of the Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HaCaT cell line (Human keratinocytes) and DMEM (Dulbecco’s Modified Eagle Medium) for wound healing action using scratch assay (48 hours) against positive control i.e., recombinant Human Epidermal Growth Factor (Hu-EGF, 30 ng/mL). In vitro scratch assay monitored proliferation and migration of keratinocytes in the scratched wounded area using WimScratch Image analysis software. The results showed that the Biofield Energy Treated DMEM group have significantly higher percentage of keratinocytes migration i.e., 35.4%, while no migration was monitored in the Biofield Energy Treated HaCaT cells as compared with the baseline control group. Additionally, the percentage of scratch area was significantly decreased by 7.3% in the Biofield Energy Treated DMEM, while an increased scratched area was reported by 14% in the Biofield Energy Treated cells. Hence, the results using scratch assay showed a promising scientific approach to differentiate the Biofield Energy Treatment on cells or media for their wound healing potential. The data concluded that The Trivedi Effect® has the significant capability in wound healing activity in DMEM as compared with the HaCaT cell line with respect to the cell migration and reduced wound scratched area. Consciousness Energy Healing Treatment (The Trivedi Effect®) can be used as a complementary and alternative approach to improve the cellular migration and proliferation and can be used in psoriasis, seborrheic dermatitis, skin cancer, rashes from bacterial or fungal infections as anti-wrinkling, skin-whitening, anti-ageing, and rejuvenating agent.
Category: Biochemistry

[9] viXra:1902.0133 [pdf] submitted on 2019-02-08 04:28:58

Evaluation of the Effect of Consciousness Energy Healing Treatment on Physicochemical and Thermal Properties of Withania Somnifera

Authors: Alice Branton, Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Snehasis Jana
Comments: 11 Pages.

Ashwagandha root extract is a well-known popular health supplement, which is used in many nutraceutical and pharmaceutical formulations for the prevention and treatment of various diseases. This study was performed to evaluate the influence of the Trivedi Effect on the physicochemical and thermal properties of ashwagandha root extract using modern analytical techniques. One part of the test sample was considered as the control sample (no Biofield Energy Treatment), where as the second part was treated with Biofield Energy remotely by a famous Biofield Energy Healer, Alice Branton and was labelled as the treated sample. The particle size values were significantly decreased by 36.96%(d10), 20.26%(d50), 8.95%(d90), and 11.25%{D(4,3)} thus the specific surface area was significantly increased by 27.85% in the treated sample compared to the control sample. The evaporation temperature and latent heat of evaporation of the treated sample were significantly decreased by 14.37% and 84.09%, respectively compared with the control sample. The total weight loss was increased by 11.37%; however, the residue amount was 73.67% less in the treated sample compared with the control sample. The Consciousness Energy Healing Treatment created a new form of ashwagandha root extract which can be used for designing new nutraceutical/pharmaceutical formulations for the treatment of insomnia, hypoglycemia, Huntington's, Alzheimer's, and Parkinson's disorders, etc.
Category: Biochemistry

[8] viXra:1902.0131 [pdf] submitted on 2019-02-07 05:14:14

Impact of the Trivedi Effect®-Energy of Consciousness Healing on Growth and Yield of the Plant Stem Cells Culture

Authors: Alice Branton, Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Snehasis Jana
Comments: 5 Pages.

The objective of the present study was to evaluate the effect of Biofield Energy Healing Treatment on the plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for their yield on day 7. All the three plant callus and MS liquid media were divided into two parts, one part was received Biofield Energy Treatment (known as The Trivedi Effect®-Energy Of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Alice Branton through the Healer’s unique Energy Transmission process remotely and defined as Biofield Energy Treated group. Another part did not receive any treatment and defined as the control group. On day 7 after the treatment, the flasks were observed and visualized under the phase contrast microscope for monitoring the cell growth and photomicrographs were captured at 200X magnification along with the wet weight of the callus cultures. Experimental data suggested that all the three plants viz. Mandukparni, Katsarika, and Amla callus showed significantly improved growth rate after seven days as compared with the respective untreated groups. The growth of the callus (weight in mg) of Mandukparni, Katsarika and Amla callus after 7 days of the Biofield Energy Treatment was 289.7, 348.5, and 512 mg respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika, and Amla was 3.3%, 7.7%, and 79.6%, respectively as compared with the untreated group. Hence, the results indicated that there was a significant growth observed after The Trivedi Effect®-Energy of Consciousness Treatment in the tested plant stem cells. It can be concluded that The Trivedi Effect® would be used as a complementary and alternative treatment to maximize most valuable natural products yield and quality best quality such as herbal medicinal plants, food and dietary supplements, flavoring and coloring agents, cosmetics and fragrance products, and many more.
Category: Biochemistry

[7] viXra:1902.0092 [pdf] submitted on 2019-02-06 04:14:43

Estimation of Collagen Synthesis in Human Dermal Fibroblast Cells: an Impact of Consciousness Energy Healing Treatment

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, Snehasis Jana
Comments: 7 Pages.

Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p≤0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line.
Category: Biochemistry

[6] viXra:1902.0086 [pdf] submitted on 2019-02-05 06:24:33

The Energy of Consciousness Healing Treatment: Impact on Physicochemical and Thermal Properties of L-Tryptophan

Authors: Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
Comments: 12 Pages.

L-tryptophan is an essential amino acid that helps in growth in infants and aids nitrogen balancing in adults, etc. This study helps in evaluating the impact of the Trivedi Effect® on the physicochemical and thermal properties of L-tryptophan with the help of various analytical techniques. One part of L-tryptophan sample didn’t receive any treatment and was called the control sample, while the other part was given the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and named as the treated sample. The particle size values were significantly altered by -43.95%(d10), -6.28%(d50), 9.97%(d90), and 10.36%[D(4,3)]; thus, the specific surface area was significantly increased by 51.61% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from -61.17% to 385.06% and -48.52% to 3.76% respectively, however, the average crystallite size was significantly reduced by 26.03% in the treated sample compared to the control sample. The total weight loss was significantly increased by 17.35%; additionally, the residual amount was significantly reduced by 83.95% in the treated sample compared to the control sample. The ΔHfusion was significantly increased by 10.43% in the treated sample compared to the control sample. Thus, the Biofield Treatment produced the novel polymorph of L-tryptophan that might give better solubility, bioavailability, and thermal stability in nutraceutical/pharmaceutical formulations with and an improved drug profile.
Category: Biochemistry

[5] viXra:1902.0085 [pdf] submitted on 2019-02-05 06:25:04

Evaluation of the Effect of Consciousness Energy Healing Treatment on the Physicochemical and Thermal Properties of Selenium

Authors: Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
Comments: 10 Pages.

Selenium is an essential micronutrient required for healthy metabolism, as well as prevention, and treatment of selenium deficiency diseases. The experiment aimed to evaluate the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of selenium using modern analytical techniques. The selenium sample was divided into two parts, one part of the test sample was called the control sample, while the second part of the test sample received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak, and was called the treated sample. The particle size values were significantly decreased by 37.69% (d10), 14.36% (d50), 4.31% (d90), and 11.58% [D(4,3)], hence, the specific surface area was significantly increased by 33.64% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from 5.23% to 100% and 75% to 111.7%, respectively; whereas 7.81% significantly decreased the average crystallite size in the treated sample than the control sample. The latent heat of fusion of the treated sample was significantly increased by 12.37% compared with the control sample. The results suggested that the Trivedi Effect® might generate a new polymorphic form of selenium which would offer better solubility, bioavailability and be thermally more stable compared with the control sample. The Biofield Treated selenium would be more useful to design novel nutraceutical/pharmaceutical formulations and might offer an enhanced therapeutic response against cardiovascular disease, cancer, neuromuscular disorders, diabetes, stress, aging, male infertility, viral diseases, degenerative ailments, etc.
Category: Biochemistry

[4] viXra:1902.0057 [pdf] submitted on 2019-02-04 04:30:30

The Impact of Biofield Energy Treated Vitamin D3 on the Structural Crosslinks, Maturation, and Bone Mineralization in Human Bone Osteosarcoma Cells (MG-63)

Authors: Alice Branton, Mahendra Kumar Trivedi, Dahryn Trivedi, Gopal Nayak, Snehasis Jana
Comments: 8 Pages.

The objective was to investigate the potential of Biofield Treated vitamin D3 and DMEM on bone markers. The test items (TIs) were separated into two parts. One part of each sample was received Consciousness Energy Treatment by Alice Branton and termed as Biofield Treated (BT) samples, while other parts of each sample denoted as untreated TI (UT). MTT data found TIs were safe and nontoxic at tested concentrations. ALP was significantly elevated by 390% and 200% in UT-DMEM + BT-TI at 0.1 and 1µg/mL, respectively; while, increased by 135.29% in BT-DMEM + BT-TI at 0.1µg/mL than UT-DMEM + UT-TI. ALP was significantly increased by 106.34%, 138.47%, and 212.68% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Collagen was significantly increased by 288.68% and 106.96% in UT-DMEM + BT-TI and BT-DMEM + BT-TI, respectively at 10µg/mL than untreated. Further, collagen was significantly increased by 435.78% and 139.72% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 50µg/mL than untreated. Percent of bone mineralization was significantly increased by 299.13%, 239.91%, and 218.77% in BT-DMEM + BT-TI at 0.1, 1, and 10µg/mL, respectively than untreated. Besides, percent of bone mineralization was distinctly increased by 192.51% in BT-DMEM + UT-TI at 10µg/mL than untreated. Overall, Biofield Treated vitamin D3 remarkably improved bone parameters in MG-63 cells and could be an alternative to vitamin D3 deficiency and used various bone disorders viz. low bone density, osteoporosis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
Category: Biochemistry

[3] viXra:1902.0056 [pdf] submitted on 2019-02-04 04:31:05

Evaluation of The Trivedi Effect® - Consciousness Energy Healing Treatment on the Growth and Yield of Plant Stem Cells

Authors: Dahryn Trivedi, Mahendra Kumar Trivedi, Alice Branton, Gopal Nayak, Snehasis Jana
Comments: 7 Pages.

This study investigated the effect of the Consciousness Energy Healing (The Trivedi Effect®) Treatment on the plant stem callus of Mandukparni (Centella asiatica), Katsarika (Baleria cristata), and Amla (Phyllanthus emblica). Each stem cell callus and MS liquid media were divided into two parts. One part of each was denoted as control. The other part was received the Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was defined as the Biofield Energy Treated sample. The weight of mandukparni callus was significantly increased by 8.5% in Biofield Energy Treated group (304.5 mg) compared to control group (280.6 mg). Furthermore, weight of katsarika stem cell callus was significantly increased by 38.6% in Biofield Energy Treated group (448.7 mg) compared to control (323.7 mg). The weight of amla stem cell callus was significantly raised by 41.4% in the Biofield Energy Treated group (403 mg) compared to control group (285 mg). Overall, the data suggest that there was a significant growth and yields of plants callus after treatment with the Biofield Energy. Therefore, The Trivedi Effect® - Consciousness Energy Healing approach could be useful for the production of more active constituents present in the three plants callus in a cost-effective manner and can be useful for food and dietary supplements, flavoring and coloring agents, cosmetics and fragrance products.
Category: Biochemistry

[2] viXra:1902.0019 [pdf] submitted on 2019-02-01 23:26:26

Assessment of Energy of Consciousness Healing on Cell Growth of Plant Stem Cells Callus Culture

Authors: Gopal Nayak, Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Snehasis Jana
Comments: 9 Pages.

The present study was aimed to evaluate the effect of Consciousness Energy Healing Treatment on plant callus of Mandukparni (Centella asiatica), Katsarika (Barleria cristata), and Amla (Phyllanthus emblica) in Murashige & Skoog liquid medium (MS) for its growth and yield on day 7. The plant callus of all the three plants were divided into two parts, one part received Biofield Energy Treatment remotely (known as The Trivedi Effect®-Energy of Consciousness Healing Treatment) by a renowned Biofield Energy Healer, Gopal Nayak and denoted as Biofield Energy Treated group, while another part did not receive any treatment and defined as control. Plants callus were observed on day 7 after treatment and visualized under phase contrast microscope for cell growth, captured photomicrographs along with wet weight compared with day 0. Results suggested that three plants viz. Mandukparni, Katsarika, and Amla callus after 7 days showed a significant improved growth rate as compared with the respective untreated groups. The weight of the callus growth (in mg) after Biofield Energy Treatment among Mandukparni, Katsarika, and Amla callus on day 7 was 630, 782, and 920 mg, respectively. However, the percentage increase in weight of the callus in Mandukparni, Katsarika and Amla was 11.1%, 52.4%, and 68.5%, respectively than untreated. Hence, results concluded a significant growth in callus weight after Biofield Energy Treatment, which suggested that it could be used as complementary and alternate approach in order to produce most high-value phytoconstituents from plant callus that can be used for making various drugs, food flavoring and colouring agents.
Category: Biochemistry

[1] viXra:1902.0014 [pdf] submitted on 2019-02-02 04:51:54

Assessment of Biofield Energy Healing Based Vitamin D3 Effects on Bone Health Parameters Using Human Osteoblast Cell Line (MG-63)

Authors: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Snehasis Jana
Comments: 9 Pages.

Poor bone health is the primary health issue, which leads to significant health problems, stress and worsening the patients' quality of life. The potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 as a test item (TI) and DMEM on MG-63 cells was investigated. The test items were treated with The Trivedi Effect® by Mahendra Kumar Trivedi and divided as Biofield Energy Treated (BT) and untreated (UT) test items. An increase in ALP activity, collagen levels, and bone mineralization was considered as the biomarker for bone health. MTT data showed that the test samples observed nontoxic in the tested concentrations. The level of ALP was significantly increased by 832.9% and 209.4% in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively at 10 µg/mL, while 222.9% increase in the BT-DMEM+BT-TI at 1 µg/mL compared to the untreated group. Collagen was significantly increased by 487.7% and 544.5% in the BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 100 µg/mL, while 116.2% at 1 µg/mL in UT-DMEM+BT-TI compared to the untreated group. Moreover, the percent of bone mineralization was significantly increased in the UT-DMEM+BT-TI and BT-DMEM+UT-TI groups by 344.9% and 149.7%, respectively at 50 µg/mL, while 183.6% in the BT-DMEM+BT-TI group at 100 µg/mL compared to the untreated group. Thus, the role of Biofield Energy Treated vitamin D3 and DMEM in order to control osteoblast function and its direct effects on bone mineralization can be used to improve bone disorders.
Category: Biochemistry