Physics of Biology

Design of an Oral Malaria Vaccine Candidate Using Yeast - Expressed CTB-MSP-TOS Fusion

Authors: Hyunho Shin
Comments: 4 Pages.

Malaria remains a significant global health challenge. This study describes the construction and expression of a fusion protein for a malaria vaccine candidate, combining the cholera toxin B subunit (CTB) with two antigens: Merozoite Surface Protein (MSP) and Transferrin-Binding Outer Surface Protein (TOS). The fusion protein, joined by GSGG linkers to maintain functional independence, was expressed in Saccharomyces cerevisiae and subsequently heat-dried to ensure yeast cell inactivation while preserving antigen integrity. This study outlines the experimental design, vector construction, protein expression, and antigen preservation methods, laying the groundwork for further characterization and testing.
Category: Physics of Biology